Stabilization of a Model Formalinized Protein Antigen Encapsulated in Poly(lactide‐co‐glycolide)‐Based Microspheres

Stabilization of a model formalinized protein antigen encapsulated in poly(lactide-co-glycolide)-based microspheres.

A formaldehyde-mediated aggregation pathway (FMAP) has been shown to be primarily responsible for the solid-state aggregation of lyophilized formalinized protein antigens [e.g., tetanus toxoid (TT) and formalinized bovine serum albumin (f-BSA)] in the presence of moisture and physiological temperature. Coincorporation of the formaldehyde-interacting amino acid, histidine, strongly inhibits the ...

Sonochemical synthesis of liquid-encapsulated lysozyme microspheres.

Liquid-encapsulated lysozyme microspheres were successfully synthesized using a sonochemical method. The encapsulation of four different liquids, namely, sunflower oil, tetradecane, dodecane and perfluorohexane on the formation, stability and morphology of the lysozyme microspheres was studied. Among the four different liquids used for encapsulation, perfluorohexane-filled microspheres were fou...

A method to tune the shape of protein-encapsulated polymeric microspheres

Protein encapsulation technologies of polymeric microspheres currently in use have been optimized to effectively protect their "protein cargo" from inactivation occurring in biological environments, preserving its bioactivity during release up to several weeks. The scenario of protein delivery would greatly benefit by strategies enabling the production of non-spherical particles. Herein we repo...

Immunogenicity of vesicular PorA formulations encapsulated in biodegradable microspheres

Drug encapsulated aerosolized microspheres as a biodegradable, intelligent glioma therapy.

The grim prognosis for patients diagnosed with malignant gliomas necessitates the development of new therapeutic strategies for localized and sustained drug delivery to combat tumor drug resistance and regrowth. Here we introduce drug encapsulated aerosolized microspheres as a biodegradable, intelligent glioma therapy (DREAM BIG therapy). DREAM BIG therapy is envisioned to deliver three chemoth...